Functions such as
flux_balance_analysis internally create a JuMP model out of the
MetabolicModel, and run the optimizer on that. To be able to make some modifications on the JuMP model before the optimizer is started, most of the functions accept a
modifications argument, where one can list callbacks that do the changes to the prepared optimization model.
The callbacks available in COBREXA.jl include functions that may help with tuning the optimizer, or change the raw values in the linear model, such as:
Compared to the variant system and the model wrappers, optimizer modifications are slightly more powerful (they can do anything they want with the optimizer!), but do not compose well – it is very easy to break the semantics of the model or erase the previous changes by carelessly adding the modifications.
Here, we show how to construct the modifications. Their semantics is similar to the variant-generating functions, which receive a model (of type
MetabolicModel), and are expected to create another (modified) model. Contrary to that, modifications receive both the
MetabolicModel and a JuMP model structure, and are expected to cause a side effect on the latter.
A trivial modification that does not do anything can thus be written as:
change_nothing() = (model, opt_model) -> println("Not touching anything.")
and applied as:
flux_balance_analysis(model, GLPK.Optimizer, modifications=[change_nothing()]) flux_variability_analysis(model, GLPK.Optimizer, modifications=[change_nothing()])
At the call time of the modifier function,
opt_model is usually the model that was returned from
make_optimization_model – refer to the function for actual model layout. The function can freely change anything in that model.
For demonstration, we show how to implement an impractical but illustrative modification that adds an extra constraint that makes sure that all fluxes sum to a certain value:
using JuMP add_sum_constraint(total::Float64) = (model, opt_model) -> begin v = opt_model[:x] # retrieve the variable vector @constraint(opt_model, total, sum(v) == total) # create the constraint using JuMP macro end
The modification can be used at the expectable position:
v = flux_balance_analysis_vec( load_model("e_coli_core.xml"), GLPK.Optimizer, modifications = [add_sum_constraint(100.0)]) sum(v) # should print ~100.0